Iq sybr green supermix bio rad
WebJul 7, 2008 · Also used was 1× iQ™ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) containing 20 mM Tris–HCl, pH 8.4, 50 mM KCl, 3 mM MgCl 2, 200 μM dNTPs, 25 U/ml iTaq DNA polymerase, unspecified amount of SGI, 20 nM fluorescein and unspecified stabilizers and 1× QuantiTect SYBR Green PCR kit (QIAGEN GmbH, Hilden, Germany) … WebThe proprietary buffer and stabilizers have been optimized exclusively for SYBR Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. This supermix provides the highest level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts.
Iq sybr green supermix bio rad
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WebJul 24, 2024 · Quantitative real-time PCR (qPCR) was performed using iQ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) on a Bio-Rad iCycler using β-actin as control, and data were analyzed using the ∆∆ C t method . All primer sequences used in this study are listed in Table 1. WebiQ™ Supermix, 170-8860 or iQ™ SYBR® Green Supermix, 170-8880 Pipette tips, aerosol barrier tips Such as: ... Bio-Rad Laboratories 2000 Alfred Nobel Drive, Hercules, CA 94547 510-741-1000 4106228 Rev A 4106228A.qxd 01/02/2003 1:13 PM Page 2. Title: 4106228A.qxd Created Date:
WebJun 4, 2016 · The most commonly used methods for quantitative polymerase chain reaction (qPCR) are based on non-specific SYBR green chemistry and specific Taqman probe chemistry [ 3 ]. Intercalating dyes, which bind double-stranded (ds) DNA with high efficiency in the reaction, are most commonly used. WebiQ™ SYBR ® Green Supermix Catalog # Supermix Volume Kit Size 170-8880 2.5 ml (2 x 1.25 ml vials) 100 x 50 µl reactions 170-8882 12.5 ml (10 x 1.25 ml vials) 500 x 50 µl reactions …
WebItem iQ™ SYBR ® Green Supermix. Company Bio-Rad. Catalog Number 170-8882. WebPerfeCTa SYBR Green FastMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Product Kit Size Order Info PerfeCTa SYBR Green FastMix for IQ Request Sample 1250 x 20 μL rxns (10 x 1.25 mL) Order (95071-012) 5000 x 20 μL rxns (1 x 50 mL) Order (95071-05K)
WebJun 28, 2016 · RNA (2 µg) was used for first-strand cDNA synthesis with reverse transcriptase (Promega Corporation, Madison, WI, USA), according to the manufacturer's protocol. cDNA was amplified by qPCR in a CFX96 Touch Real-Time PCR Detection system with CFX Manager 3.0 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA) using iQ …
WebBriefly, 25 μL of reaction mixture containing 1 X iQ™ SYBR Green Supermix (Bio-Rad, Mississauga, ON), 10 ng of cDNA and 0.2 μM of specific primers to detect PI3K, (Forward: 3′-GTCTCCTCTTGCCCCTG AT-5′ and reverse: 5′-TCGAAATGCCAACCGTAAT-3′), Aminocyclopropane-1-carboxylate oxidase 1 (ACO1, forward: 5′ … how to start an italian ice businessWeba SYBR Green SuperMix for iQ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitativ e PCR on Bio-Rad iCycler iQ ® systems. The proprietary buffer and stabilizers have been optimized exclusively for SYBR Green I qPCR how to start an isp companyWeba SYBR Green SuperMix 95054 -500, 95054-02K Bio-Rad CFX96 ™ , CFX384 ™ , Opticon™, MiniOpticon™, Chromo4™ Cepheid Smart Cycler ®;Qiagen/Corbett Rotor-Gene Eppendorf Mastercycler ® ep realplex Roche Applied Science LightCycler ® 480 . Components . PerfeC. T. a SYBR Green SuperMix (2X): 2X reaction buffer containing optimized ... react book pdf downloadWebSYBR ® Green Supermix: iQ™ SYBR ® Grow Supermix EpiQ™ Chromatin: SYBR ® Green Supermix: SsoAdvanced Universal Probes Supermix: iTaq Universal Queries Supermix: iQ Supermix and iQ Multiple Powermix: iTaq™ Universal SYBR ® Green One-Step Equipment: iTaq Universal Acoustic One-Step Kit: Bio-Rad: CFX96™, CFX96 Touch™, CFX384 ... how to start an iv infusion companyWebSupplier: Bio-Rad. iTaq™ Universal SYBR® Green Supermix is a 2x concentrated, ready-to-use master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR … react booking systemWebPerfeCTa SYBR ® Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). react bookingWebThis supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Use of iQ™ SYBR® Green supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude. react bookmark