WitrynaMitotic kinetochores are initially captured by dynamic microtubules via a ‘search-and-capture’ mechanism. The microtubule motor, dynein, is critical for kinetochore capture as it has been shown to transport microtubule-attached chromosomes towards the spindle pole during early mitosis. The microtubule-binding Ndc80 complex that is recruited to … WitrynaErba Diagnostics human crest autoimmune serum Human Crest Autoimmune Serum, supplied by Erba Diagnostics, used in various techniques. Bioz Stars score: 85/100, …
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WitrynaACA Immunovision HCT-0100 1:5000 Pericentrin Abcam Ab4448 1:750 Plk1 Santa Cruz SC-17783 1:1000 1:1000 Flag Sigma F1804 1:500 dsRed Clontech 632496 1:1000 Cyclin B1 BD Biosciences 554177 1:2000 pS170-Cyk4 Home brewed (Burkard et al., 2009) 1:500 Mklp1 Santa Cruz SC-867 1:350 ... Witryna2 Background: The main role of the cell cycle is to enable error-free DNA replication, chromosome segregation and cytokinesis. One of the best characterised checkpoint pathways is the spindle assembly checkpoint, which prevents anaphase onset until the grandview of westover hills
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WitrynaArticle Title: A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint Journal: Genes & Development doi: 10.1101/gad.184184.111 WitrynaInactivation of the BLM helicase leads to the genome instability disorder known as Bloom’s syndrome (BS). Cells derived from BS patients display an increased frequency of sister chromatid exchanges (SCEs) and genome instability ( Chaganti et al. 1974 ). These SCEs arise through the actions of three structure-selective endonucleases, … Witryna29 paź 2016 · CREST serum (ImmunoVision, HCT-0100). 18. Fluorescent secondary antibodies . 19. 4′,6-diamidino-2-phenylindole (DAPI). 20. Plastic coverslip. Fold in the middle and use for antibody incubation. 21. Shandon Cytospin centrifuge (Thermo Scientific). 22. Shandon Double Cytoslides microscope slides (Thermo Scientific). 23. chinese takeaway in morley